Fig. 1.

Importance of glutaminase in the growth of NSCLC. a NSCLC cells (H1299, H292, SPC-A1, A549, and H23) were cultured in RPMI 1640 with 10% FBS in the presence or absence of glutamine for 6 days; and normal human bronchial epithelial (HBE) cells were cultured in Airway epithelial cell basal medium in the presence or absence of glutamine for 6 days before cells were trypsinized and counted. Data represent the average of three independent experiments (mean ± SD). ***P < 0.001, ns: P > 0.05. b–d NSCLC cells were cultured in RPMI 1640 with 10% FBS and HBE cells were cultured in Airway epithelial cell basal medium, cells were transfected with either control siRNA or GAC siRNAs. At the indicated times, cells were fixed in 3.7% formaldehyde and stained with 0.1% crystal violet. Dye was extracted with 10% acetic acid and the relative proliferation was assessed from the increase in absorbance at 595 nm. Data represent the average of three independent experiments (mean ± SD). ***P < 0.001, ns: P > 0.05 (top figures). The knockdown efficiency was determined by western blotting using anti-GAC antibody (bottom figures). e NSCLC cell lines (H23, H1299, H292, A549, and SPC-A1) were cultured in RPMI 1640 with 10% FBS in the presence or absence of 10 μM 968 for 6 days; HBE cells were cultured in Airway epithelial cell basal medium in the presence or absence of 10 μM 968 for 6 days, then cells were trypsinized and counted. Data represent the average of three independent experiments (mean ± SD). ***P < 0.001, ns: P > 0.05