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. 2018 Jun 7;70(5):801–813.e6. doi: 10.1016/j.molcel.2018.04.016

Figure 3.

Figure 3

Measurement of Broken Fraction

(A) Schematic view of the ligation mediated (LM)-PCR assay to detect the broken ends after DSB induction. See text for explanation.

(B) Representative agarose gel of the LM-PCR products of a time series. The expected product is 205 bp in size.

(C) The broken fraction measured as band intensities (data from 8 LM-PCR experiments from 4 independent time series; values are a.u.; mean ± SD). Solid blue line shows an ODE curve fit to the LM-PCR data to determine τ (blue shading, mean ± SD).

(D and E) Same as (B) and (C) but with primers 2 and 3 that do not span the break site. The expected product is 163 bp in size. Values are in a.u., mean ± SD, based on 7 PCR experiments from 3 independent time series.