Figure 5.

MMEJ Has Slower Repair Kinetics Than C-NHEJ

(A) Kinetic model of Cas9-induced DSB repair assuming that each type of indel is generated with a specific repair rate.

(B) +1 insertion and −7 deletion accumulation in a representative time series. Dashed lines show a Gompertz sigmoid fit.

(C) Same measurements as in (B) with the multi-indel ODE model fit (solid lines) for the +1 (top) and −7 (bottom) indels.

(D) Cartoon representation of hypothesized time dependency of the k₊₁ and k₋₇ rates added to the fitted model in (E).

(E) Time-dependent ODE model fit of the +1 insertion (top) and −7 deletion (bottom) as proposed in (D).

(F) Rate constants without (black) and with (gray) NU7441. The total mutagenic repair rate is represented by k_m; rate constant for the +1 insertion is k₊₁ and rate constants for the −7 deletion are k_{−7,t = 0hr} and k_{−7,t = 60hr} at the start and end of the time series, respectively. n indicates the number of time series. Asterisks indicate p values according to Wilcoxon test: ^∗p < 0.05; ^∗∗p < 0.005; ^∗∗∗p < 0.0005.

(G) Broken fraction measurements in presence of NU7441 of 2 independent time series, similar to Figures 3B and 3C.