REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Cas9 Monoclonal Antibody (7A9)	Thermo Fisher Scientific	MA5-23519
Monoclonal Anti-γ-Tubulin Clone GTU-88	Sigma Aldrich	T6557
IRDye 800CW goat anti-mouse IgG	Li-Cor	926-32210
Bacterial and Virus Strains
Escherichia coli α-Select Silver Competent Cell	Bioline	BIO-85026
Lenti virus	N/A	N/A
Biological Samples
K-562 Homo sapiens bone marrow chronic myelogenous leukemia (CML)	ATCC	CCL-243
Chemicals, Peptides, and Recombinant Proteins
Shield-1	Aobious	AOB1848
NU7441	Cayman Chemical	503468-95-9
T4 ligase 5 U/μL	Roche	10799009001
Phusion High Fidelity DNA polymerase	Thermo Fisher Scientific	F530
MyTaq Red mix	Bioline	BIO-25043
Cas9 Nuclease, S. pyogenes	NEB	M0386S
Critical Commercial Assays
CellTiter-Blue Cell Viability Assay	Promega	G8080
Isolate II Genomic DNA Kit	Bioline	BIO-52067
Isolate II PCR and Gel Kit	Bioline	BIO-52060
MEGAclear Transcription Clean-Up Kit	Thermo Fisher Scientific	AM1908
RiboMAX	Promega	P1300
Pierce BCA protein assay	Thermo Fisher Scientific	23225
Deposited Data
Raw Imaging Files	This study, Mendeley Data	http://dx.doi.org/10.17632/wg4ssg7pfw.1
Sequence files	This study, GEO	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE113129
Experimental Models: Cell Lines
K562#17	This study	N/A
Oligonucleotides
sgRNA oligos (see Table S1 for sequences)	IDT/Invitrogen	N/A
Primers (see Table S2 for sequences)	IDT/Invitrogen	N/A
dsDamID adaptor	Vogel et al., 2007	N/A
Recombinant DNA
pX330-U6-Chimeric_BB-CBh-hSpCas9	Cong et al., 2013	Addgene 42230
pLenti-Cas9-T2A-Neo	Prahallad et al., 2015	gift of Dr. B. Evers, NKI
pPTuner IRES2	Clontech	632168
DD-Cas9	This study	N/A
pBlue-sgRNA	This study	N/A
Software and Algorithms
R Studio- Open source edition	Rstudio	https://www.rstudio.com/
Sequence data analyses	This paper	https://github.com/vansteensellab/DSB_Repair_Kinetics
Kinetics modeling	This paper	https://github.com/vansteensellab/DSB_Repair_Kinetics
TIDE software	https://tide.nki.nl	N/A
Other
Cs source Gammacell 40 Exactor	Best Theratronics	N/A
ChemiDoc Imaging Systems	BioRad	N/A
BD FACSCalibur	BD Biosciences	N/A
Odyssey scanner	Li-Cor	N/A