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. 2018 Feb 23;26(5):1206–1214. doi: 10.1016/j.ymthe.2018.02.017

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The 7B5 T Cell Clone Recognizes HA-2-Expressing EBV-LCLs and Primary AML Samples

(A) T cell clones 7B5 and HA2.27 (5,000/well) were cocultured with several EBV-LCLs at a stimulator-to-responder (S:R) ratio of 6:1. Controls included EBV-LCLs from the patient (LCL MRJ, HLA-A*02:01^pos HA-2^pos) and donor (LCL IZA, HLA-A*02:01^pos HA-2^neg) we isolated T cell clone HA2.27 from, as well as HLA-A*02:01^neg controls LCL-MSF and LCL-PSU. After overnight incubation, supernatants were harvested, and the concentration of IFN-γ was measured by ELISA. Depicted values are the average of duplicate measurements ± SD. (B) T cell clones 7B5 and HA2.27 (5,000/well) were cocultured with HLA-A*02:01^pos HA-2^neg primary AML sample (AML1), HLA-A*02:01^pos HA-2^pos primary AML samples (AML2,-3,-4), and HLA-A*02:01^pos HA-2^neg fibroblasts (FB) and keratinocytes (KC) at an S:R ratio of 6:1. After overnight incubation, supernatants were harvested, and the concentration of IFN-γ was measured by ELISA. Depicted values are the average of duplicate measurements ± SD.