Figure 1.

CLN6 Is More Highly Expressed in Bipolar Cells Than in Photoreceptors in Human and Mouse Retina

Staining for CLN6 (green) and PKCα (red) on human retinal section showing high levels of CLN6 in the INL and lower levels in the ONL with (A) and without (B) DAPI nuclear counterstaining in blue. Gray boxes show areas of higher-magnification images. Scale bar, 50 μm. Single confocal image reveals (B’) CLN6⁺ rod bipolar cells (arrows) and (B”) CLN6⁺ cells in the ONL. Scale bar, 25 μm. (C) Cln6 expression level in adult mouse bipolar cells relative to photoreceptors after normalization for β-actin (mean ± SEM; n = 3 samples of 3–5 pooled retinas each; not significant, Mann-Whitney U test). (D) Absolute expression levels of Cln6 and Peripherin2 in adult wild-type mouse retinas (means ± SEM). Peripherin2, n = 3 mice; Cln6^nclf, n = 4 mice. (E) Time course of the expression level of Cln6 in wild-type mouse retinas relative to adult levels, normalized for β-actin (mean ± SEM; Kruskall-Wallis, **p < 0.01 relative to P21; n = 2–4 mice per time point).