Figure 3.

AAV 7m8 Vector Transduced Mouse Bipolar Cells Efficiently

(A) Section of wild-type mouse eye 3 weeks post-intravitreal injection of 1 × 10¹⁰ vg 7m8.CMV.EGFP at P5. Scale bar, 250 μm. (B and C) Higher-magnification images of wild-type retinas treated with 7m8.CMV.EGFP and immunostained for PKCα (red) without (B) and with (C) the green channel shown. Scale bar, 50 μm. (C’) Single merged image of 7m8.CMV.EGFP-treated wild-type retina showing transduced PKCα⁺ rod bipolar cells. Scale bar, 25 μm. (D) Section of wild-type mouse eye 3 weeks post-intravitreal injection of 1 × 10¹⁰ vg 7m8.Grm6.EGFP at P5. Scale bar, 250 μm. (E) Higher-magnification image of 7m8.Grm6.EGFP-treated retina stained for PKCα (red). Scale bar, 50 μm. (E’) Single image demonstrating transduction of the majority of PKCα⁺ rod bipolar cells (arrows indicate non-transduced PKCα⁺ cells) and some cone ON bipolar cells (arrowheads), identified by the location of their cell bodies in the INL and synaptic termini in the inner plexiform layer (IPL). Scale bar, 25 μm. (F) Quantification by flow cytometry of the proportion of rod bipolar cells transduced by 7m8 at P5–P6 (mean ± SD; n = 7 eyes).