Figure 4.

Distribution of JR-141 in Cynomolgus Monkeys after Intravenous Administration

(A) Pharmacokinetics of JR-141 in the plasma of the monkey. JR-141 was administered intravenously at a dose of 5 mg/kg (0–8 hr, n = 4; 12 and 24 hr, n = 2). Data were plotted as mean ± SD. Detailed pharmacokinetic parameters are presented in Table S2. (B and C) Concentrations of JR-141 in the peripheral tissues (B) and the brain and spinal cord (C). The heart, kidney, liver, lung, spleen, cerebral cortex, cerebellum, hippocampus, and spinal cord were resected. JR-141 in each tissue homogenate was quantified by electrochemiluminescent immunoassay (n = 2). Bars indicate the mean. (D) Immunohistochemical analysis of the cerebellum. Arrows indicate Purkinje cells. (E) Immunohistochemical analysis of the hippocampus. Arrows indicate the pyramidal cells. The brains were resected at 8 hr after the administration. Scale bars, 20 μm.