Figure 3.

miR-223-3p Inhibits Osteosarcoma Cell Invasion, Migration, Growth, and Proliferation through the Suppression of CDH6 Production

(A and B) Invasion of 143B cells transfected with miR-223-3p or miR-223-3p plus CDH6 (A) or miR-223-3p inhibitor (B) was evaluated using a Matrigel invasion chamber. The invaded cells were fixed and stained with crystal violet (A and B left images). The scale bar represents 100 μm. (C and D) Wound healing assays were conducted in 143B cells transfected with miR-223-3p or miR-223-3p and CDH6 (C) or miR-223-3p inhibitor (D). Cell migration was measured 24 hr after the cell layers were scratched. (E and F) 143B cells expressing miR-223-3p or miR-223-3p and CDH6 (E) and 143B cells transfected with miR-223-3p inhibitor (F) were cultured in regular medium. At the specified times, cell numbers were determined with the CCK-8 assay. The representative immunoblot shows CDH6 expression. (G and H) 143B cells transfected with miR-223-3p (G) or miR-223-3p inhibitor (H) were plated and assayed for colony formation. Representative images show colonies in plates (left panels). Each bar represents the mean ± SD of at least three independent experiments performed in triplicate. *p < 0.05; **p < 0.01.