Fig. 1.

Endogenous and ectopic expression of SLC12A7 in ACC cells. a Relative messenger RNA (mRNA) expression levels in adrenocortical carcinoma cells lines SW-13, SW-13/V, SW-13/S, and NCI-H295R by real-time quantitative polymerase chain reaction were determined. Expression levels were significantly higher in SW-13/S and NCI-H295R cells compared to SW-13 and SW-13/V cells (*, p < 0.05, one-way ANOVA). Gene expression analysis was performed in triplicate. Horizontal bar, mean; Error bars, standard error of mean. b Western immunoblot of SLC12A7 in SW-13, SW-13/V, and SW-13/S cells. Protein expression was higher in SW-13/S cells compared to SW-13 and SW-13/V cells. β-actin served as a loading control. Western blot analysis was performed in duplicate. c Relative mRNA expression levels were determined in NCI-H295 cells undergoing RNAi gene silencing by three siRNAs (siRNA-A, siRNA-B, and siRNA-C) targeting SLC12A7 and scrambled siRNA. Significantly lower expression levels were noted in siRNAs B and C transfected cells compared to parent cells (*, p < 0.05, one-way ANOVA). Horizontal bar, mean; Error bars, standard error of mean. Gene expression analysis was performed in triplicate