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. 2018 Jun 8;16:27. doi: 10.1186/s12964-018-0243-0

Fig. 4.

Fig. 4

a One hundred thousand cells of NCI-H295R cells were allowed to migrate through a Boyden chamber. Decreased migration activity was noted in NCI-H295R cells undergoing SLC12A7 knock down with siRNA B duplex (*, p < 0.05, one-way ANOVA). Horizontal bar, mean; Error bars, standard error of mean. Migration assays were performed in two independent experiments using 3 wells per each experimental condition analyzed. b One hundred thousand cells of NCI-H295R cells were allowed to invade through a Matrigel layer in a modified Boyden chamber. Decreased invasion activity was noted in NCI-H295R cells undergoing SLC12A7 knock down with siRNA B duplex (*, p < 0.05, one-way ANOVA). Horizontal bar, mean; Error bars, standard error of mean. Invasion assays were performed in two independent experiments using 3 wells per each experimental condition analyzed. c Representative photomicrographs of NCI-H295R invasion undergoing siRNA knockdown are shown right