Fig. 2.
Mitochondrial function. Results are represented by mean ± SEM, comparing controls (n = 8; white bars), NM-LRRK2G2019S-mutation, (n = 6; grey bars) and PD-LRRK2G2019S (n = 7; black bars) in glucose and galactose media. a Citrate synthase levels remained constant between groups in both media. b A trend to decrease CI enzymatic activity in NM-LRRK2G2019S and PD-LRRK2G2019S was observed in glucose media, and PD-LRRK2G2019S patients harbouring the mutation increased their activity with respect to controls in galactose media (p = 0.04). c Oxygen consumption trended to reproduce the pattern of CI enzymatic activity. After exposure to galactose media, a non-significant increment in O2 consumption was observed in fibroblasts of NM-LRRK2G2019S subjects with respect to controls (p = 0.35). d Complex IV enzymatic function remained similar in all groups, showing a trend to increase in NM-LRRK2G2019S subjects with respect to controls in glucose and galactose media (p = 0.14 in both cases). e ATP levels tended to decrease in both groups in glucose media (p = 0.18 when comparing PD-LRRK2G2019S with controls). f Number of depolarized mitochondria was increased in NM-LRRK2G2019S when compared to controls in glucose media (p = 0.04), with the same trend observed for PD-LRRK2G2019S although without reaching statistical significance (p = 0.14). g Oxidative damage trended to increase in PD-LRRK2G2019S patients in both conditions when comparing them to controls. h Apoptotic rate tended to decrease in the NM-LRRK2G2019S group in both media