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COX-2 immunofluorescence in coronal sections of the ventral cervical spinal cord is similar after systemic inflammation induced by IH-1 compared to normoxia treated. Confocal images (10× magnification) show representative COX-2 staining (green) after normoxia (A, n=6) and IH-1 (B, n=6). Overlay images show phrenic motor neurons identified by CtB back-label (blue) and CD11b labeled microglia (red).
