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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Jan 31;141(6):2085–2093.e1. doi: 10.1016/j.jaci.2018.01.001

Figure 4. Knock-down of ANKRD1 enhances HSV-1 viral loads and reduces production of type I and type III IFNs in APCs.

Figure 4

Primary APCs from normal subjects (n=3) were isolated by depletion of T cells and NK cells. The cells were then transfected with scrambled siRNA duplexes and ANKRD1 siRNA duplexes. After 24 hours of incubation, HSV-1 at indicated doses was added to the cultures for an additional 24 hours of incubation. The cells were then harvested for RNA extraction and qRT-PCR. (A) ANKRD1, (B) HSV-1, (C) IFNb1 and (D) IL-29 were evaluated by qRT-PCR. (E). Primary dendritic cells from normal subjects (n=3) were isolated and transfected with siRNA duplexes for 24 hours. The cells were then stimulated with ODN 2395 for an additional 24 hours. ANKRD1, IFNb1 and IL-29 were evaluated by qRT-PCR. Data represented as mean ±SE. The data are representative of three independent experiments.