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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Exp Eye Res. 2018 Apr 13;172:152–158. doi: 10.1016/j.exer.2018.04.005

Figure 7.

Figure 7

Ex vivo wound healing model to study mouse corneal epithelial cell migration. A. Corneal epithelial cells were isolated, cultured as monolayers on fibronectin-coated cell culture dishes, scratch-wounded, and repair monitored at regular intervals under a light microscope. Wild type (AQP5+/+) showed faster wound closure than AQP5 knockout (AQP5−/−) 16 hours after wounding. Blue dashed lines: wound margin immediately after wounding. Red dashed lines: cell migration after 16 hours. B. Speed of cell migration of the wound edges of isolated corneal epithelial cells of wild type (AQP5+/+) and AQP5 knockout (AQP5−/−). Wild type (AQP5+/+) showed significantly (P<0.01) faster cell migration than AQP5 knockout (AQP5−/−). Five random sample areas were selected for quantification. Scale bar = 75μm.