Fig. 2.

The immunocytochemical analysis of mesenchymal stem cells and neural cells markers expressed by WJ fragments in control conditions; they co-cultured with untreated hippocampal slices and with hippocampal slices damaged by oxygen glucose deprivation. The nuclei were stained with Hoechst (m–p) or NuMa (o upper picture). Our results show that mesenchymal stem cell marker fibronectin (a) was expressed more extensive in the naive culture than in those after 21 days of cultivation (e) or co-culturing with hippocampal slices injured or not (i, m) by OGD. Moreover, the expression of neuronal marker NF200 (c, g, k, o bottom), oligodendrocyte marker NG2 (D,H, L) and astrocyte marker GFAP (b, f, j, n), appeared or increased after co-culture with OHC and further potentiated when OHCs had been damaged by transient OGD episode. Scale bars of 100 μm are inserted on the bottom of pictures