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. 2017 Nov 13;55(7):6021–6036. doi: 10.1007/s12035-017-0802-1

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — The quantitative immunocytochemistry analysis of number of Wharton’s jelly mesenchymal stem cells from passage 1 (a) and passage 4 (b) cultured in different cultivation conditions and comparison between number of markers expressed by cells from passages 1 and 4 (c). In both cases, mesenchymal stem cells markers fibronectin and vimentin were expressed in similar amount by each type of culture. The expression of neural markers NG2, Nestin, GFAP, NF200, TUJ1, and proliferation marker Ki67 increased after co-culturing with OHC and further increased after co-culturing with OHC damaged after OGD. The comparison showed that WJ-MSC p.1 expressed more mesenchymal stem cells markers, whereas WJ-MSC p.4 immunodetected more neural and proliferation markers in all types of the culture