Table 3.
Proteins decreased in lipid raft fractions of rats injected with GW4869 nSMase inhibitor (Inb) or saline (Sal); or antisense (AS) vs. scrambled sense (SS)
| Gene symbol | Name | Pro_Mass | AS/SS | Inb/Sal |
|---|---|---|---|---|
| Eef2 | Elongation factor 2 | 96192 | 0.082391 | 0.356 |
| Anxa6 | Annexin | 76108 | 0.105078 | 0.061913 |
| Prdx5 | Isoform cytoplasmic + peroxisomal of peroxiredoxin-5, mitochondrial | 17195 | 0.115124 | 0.253253 |
| Lonp1 | Lon protease homolog, mitochondrial | 106296 | 0.139431 | 0.267 |
| Aars | Alanine-tRNA ligase, cytoplasmic | 107522 | 0.139431 | 0.4005 |
| Hpca | Neuron-specific calcium-binding protein hippocalcin | 22527 | 0.174288 | 0.205385 |
| LOC100361259 | 60S ribosomal protein L13 | 24168 | 0.179974 | 0.492118 |
| Ctnna1 | Catenin (cadherin-associated protein), alpha 1 | 100858 | 0.18126 | 0.4984 |
| Spr | RCG56371 | 28510 | 0.185908 | 0.34176 |
| Ssrp1 | FACT complex subunit SSRP1 | 81206 | 0.185908 | 0.438154 |
| Ndrg1 | Protein NDRG | 43383 | 0.193344 | 0.167529 |
| Anxa7 | Annexin | 50272 | 0.2014 | 0.237333 |
| Cbr1 | Carbonyl reductase [NADPH] 1 | 30920 | 0.210555 | 0.248121 |
| Coro1c | Coronin | 53828 | 0.220765 | 0.328615 |
| Dpp6 | Dipeptidyl aminopeptidase-like protein 6 | 91842 | 0.2226 | 0.412211 |
| Celf2 | CUGBP Elav-like family member 2 | 54291 | 0.231018 | 0.397882 |
| Oxr1 | Oxidation resistance protein 1 | 97418 | 0.232385 | 0.323636 |
| Gng3 | Guanine nucleotide-binding protein subunit gamma | 8527 | 0.250224 | 0.294869 |
| H3f3b | Histone H3 | 15376 | 0.255078 | 0.441192 |
| A0A0G2JTG1 | Uncharacterized protein | 9382 | 0.256959 | 0.286437 |
| Park7 | Park7 protein | 23002 | 0.260142 | 0.44144 |
| Tsg101 | Tumor susceptibility gene 101 | 44221 | 0.264338 | 0.3115 |
| Fkbp1a | Peptidyl-prolyl cis-trans isomerase FKBP1A | 11972 | 0.270931 | 0.462391 |
| Frrs1l | DOMON domain-containing protein FRRS1L | 32892 | 0.271452 | 0.314986 |
| Hist1h4b | Histone H4 | 11360 | 0.27173 | 0.416595 |
| Pabpc1 | Polyadenylate-binding protein 1 | 60149 | 0.27189 | 0.408525 |
| Rpl24 | 60S ribosomal protein L24 | 17779 | 0.273329 | 0.196058 |
| Chchd6 | MICOS complex subunit Mic25 | 29592 | 0.2736 | 0.449684 |
| A0A0G2KBA1 | Uncharacterized protein | 31964 | 0.274636 | 0.319673 |
| Erp29 | Endoplasmic reticulum resident protein 29 | 28614 | 0.274636 | 0.323636 |
| Pitpna | Phosphatidylinositol transfer protein alpha isoform | 32059 | 0.276925 | 0.209412 |
| Actn3 | Actin in alpha 3, isoform CRA_a | 103575 | 0.278862 | 0.4272 |
| Lancl2 | LanC lantibiotic synthetase component C-like 2 (bacterial) | 51677 | 0.278862 | 0.4628 |
| Ube2n | Ubiquitin-conjugating enzyme E2 N | 17170 | 0.281023 | 0.331163 |
| Ndufs7 | NADH dehydrogenase (ubiquinone) Fe-S protein 7 | 24215 | 0.28196 | 0.212085 |
| Blvrb | Biliverdin reductase B (flavin reductase (NADPH)) | 22194 | 0.283219 | 0.33375 |
| Dynll2 | Dynein light chain 2, cytoplasmic | 10457 | 0.283631 | 0.366812 |
| Atp5j2 | ATP synthase subunit f, mitochondrial | 10503 | 0.283934 | 0.368729 |
| Rab7a | Ras-related protein Rab-7a | 21361 | 0.284329 | 0.440145 |
| Sdhb | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial | 32607 | 0.287714 | 0.090127 |
| Canx | Calnexin | 67612 | 0.287714 | 0.237333 |
| Pdxp | Pyridoxal phosphate phosphatase | 33380 | 0.287714 | 0.339048 |
| Cdc42 | Cell division control protein 42 homolog | 21696 | 0.292945 | 0.146051 |
| Cars | Cysteinyl-tRNA synthetase (predicted), isoform CRA_b | 86110 | 0.3021 | 0.237333 |
| Cacnb2 | Calcium channel, voltage-dependent, beta 2 subunit, isoform CRA_d | 68596 | 0.3021 | 0.237333 |
| Ppp2r5b | Protein LOC100909468 | 57746 | 0.3021 | 0.34176 |
| Lrrc7 | Leucine-rich repeat-containing protein 7 | 168690 | 0.3021 | 0.356 |
| Ddx5 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 | 69709 | 0.3021 | 0.356 |
| Atad3 | ATPase family AAA domain-containing protein 3 | 66889 | 0.3021 | 0.356 |
| Gad2 | Glutamate decarboxylase 2 | 66215 | 0.3021 | 0.356 |
| Sacm1l | Phosphatidylinositide phosphatase SAC1 | 67509 | 0.3021 | 0.356 |
| Clip2 | CAP-Gly domain-containing linker protein 2 | 115975 | 0.3021 | 0.356 |
| Aldh2 | Aldehyde dehydrogenase, mitochondrial | 56994 | 0.314688 | 0.303787 |
| Uqcr10 | Protein Uqcr10 | 7095 | 0.318671 | 0.150211 |
| Pdia3 | Protein disulfide-isomerase | 57499 | 0.3192 | 0.322415 |
| Suclg1 | Succinate-CoA ligase, GDP-forming, alpha subunit, isoform CRA_b 1 | 37935 | 0.336958 | 0.4628 |
| Pacsin1 | Protein kinase C and casein kinase substrate in neurons protein 1 | 50760 | 0.342924 | 0.201217 |
| Atp5o | ATP synthase subunit O, mitochondrial | 23440 | 0.344484 | 0.277985 |
| Btbd8 | Protein Btbd8 | 149108 | 0.345257 | 0.4005 |
| Rpl30 | 60S ribosomal protein L30 | 12947 | 0.355412 | 0.418824 |
| Idh3B | Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial | 42612 | 0.358744 | 0.459717 |
| Hnrnpab | CArG-binding factor A | 30948 | 0.359643 | 0.32752 |
| Ube2o | Protein Ube2o | 126509 | 0.36252 | 0.4272 |
| Pcdh1 | Protein Pcdh1 | 129114 | 0.36252 | 0.4272 |
| Fam49a | Family with sequence similarity 49, member A | 37704 | 0.366182 | 0.2581 |
| Cox6c2 | Cytochrome c oxidase subunit 6C-2 | 8449 | 0.368122 | 0.279335 |
| Cryab | Alpha-crystallin B chain | 20076 | 0.368664 | 0.434441 |
| Hspa5 | 78-kDa glucose-regulated protein | 72473 | 0.369559 | 0.42417 |
| Sfpq | Protein Sfpq | 75210 | 0.372804 | 0.188075 |
| Gsta1 | Glutathione S-transferase | 25360 | 0.375947 | 0.3115 |
| Rac1 | Ras-related C3 botulinum toxin substrate 1 | 24636 | 0.384326 | 0.229125 |
| Pip4k2b | Phosphatidylinositol 5-phosphate 4-kinase type-2 beta | 47633 | 0.384491 | 0.453091 |
| Dnm1l | Dynamin-1-like protein | 84369 | 0.387066 | 0.460283 |
| Ccsap | Protein Ccsap | 28584 | 0.387308 | 0.34176 |
| Cct5 | T-complex protein 1 subunit epsilon | 59955 | 0.390953 | 0.326333 |
| Ehd3 | EH domain-containing protein 3 | 60810 | 0.390953 | 0.403467 |
| Scrn1 | Secernin-1 | 46994 | 0.394043 | 0.1246 |
| Lynx1 | Ly6/neurotoxin 1 (predicted), isoform CRA_a | 13396 | 0.395996 | 0.466649 |
| P11517 | Hemoglobin subunit beta-2 | 16086 | 0.4028 | 0.165568 |
| Map4 | Microtubule-associated protein | 235075 | 0.4028 | 0.2848 |
| Hnrnpm | Isoform 2 of heterogeneous nuclear ribonucleoprotein M | 56864 | 0.4028 | 0.474667 |
| Wdr13 | Protein Wdr13 | 43571 | 0.4028 | 0.258909 |
| Epn2 | Epsin 2 | 68809 | 0.4028 | 0.474667 |
| Atp1a2 | Sodium/potassium-transporting ATPase subunit alpha-2 | 113457 | 0.415242 | 0.495857 |
| LOC684681 | Protein LOC684681 | 21304 | 0.415388 | 0.109045 |
| Pabpc4 | Polyadenylate-binding protein | 71188 | 0.42294 | 0.459355 |
| Atp4a | Sodium/potassium-transporting ATPase subunit alpha | 115674 | 0.435024 | 0.4272 |
| Mog | Myelin-oligodendrocyte glycoprotein | 28153 | 0.441366 | 0.363574 |
| Nono | Non-POU domain-containing octamer-binding protein | 55005 | 0.441531 | 0.449684 |
| Phb2 | Prohibitin-2 | 33148 | 0.444169 | 0.452486 |
| Rhob | Rho-related GTP-binding protein RhoB | 22565 | 0.447108 | 0.37024 |
| Ldha | L-lactate dehydrogenase | 36712 | 0.447414 | 0.415081 |
| Syncrip | Heterogeneous nuclear ribonucleoprotein Q | 62861 | 0.447941 | 0.41296 |
| Rtn4rl2 | Reticulon-4 receptor-like 2 | 46896 | 0.448277 | 0.260488 |
| Gstm3 | Glutathione S-transferase Yb-3 | 25835 | 0.45861 | 0.24935 |
| Atp6v1c1 | V-type proton ATPase subunit C 1 | 44044 | 0.459392 | 0.388364 |
| Palm | Paralemmin-1 | 42072 | 0.459717 | 0.356 |
| Idh3a | Isocitrate dehydrogenase [NAD] subunit, mitochondrial | 41606 | 0.462791 | 0.33802 |
| Acat1 | Acetyl-CoA acetyltransferase, mitochondrial | 45009 | 0.464422 | 0.458576 |
| Rala | Ras-related protein Ral-A | 23709 | 0.468255 | 0.4094 |
| Rpl10a | 60S ribosomal protein L10a | 24987 | 0.469334 | 0.439319 |
| Msn | Moesin | 67899 | 0.469933 | 0.263704 |
| Dlg2 | Disks large homolog 2 | 95331 | 0.474729 | 0.3204 |
| Fam49b | Fam49b protein | 37038 | 0.477949 | 0.251655 |
| Alb | Serum albumin | 70682 | 0.48336 | 0.246206 |
| Tppp | Protein Tppp | 25738 | 0.48336 | 0.362473 |
| Mapk1 | Mitogen-activated protein kinase 1 | 41648 | 0.48336 | 0.4895 |
Proteins that were reduced by more than 50% by both genetic and pharmacological inhibition of nSMase2 are listed