Fig. 7.

The effect of Aggf1 treatment on expression of downstream molecules at 24 h after subarachnoid hemorrhage (SAH). a Representative Western blot band of the downstream signaling pathway protein. b–i Densitometric quantification suggested that exogenous Aggf1 significantly upregulated the levels of PI3K, p-Akt, VE-cadherin, Occludin, and claudin-5, which were markedly reduced at 24 h post-SAH. Moreover, treatment with rh-Aggf1 significantly decreased p-NF-κB p65, TNF-α, and IL-1β, at the same time. Conversely, knockdown of endogenous Aggf1 using Aggf1 siRNA resulted in a decrease of PI3K, p-Akt, VE-cadherin, Occludin, and claudin-5 and an increase of p-NF-κB p65, TNF-α, and IL-1β. ^#P < 0.05 vs sham; *P < 0.05 vs vehicle; ^@P < 0.05 vs rh-Aggf1, vehicle, and Scr siRNA. Scr siRNA, scrambled siRNA