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The protein fold change of atrogin-1/MuRF-1 after 4 weeks of treatment. Atrogin-1/MuRF-1 proteins were detected by western blot; the grayscale ratio to GAPDH was calculated (a). Compared with that in the control, the SD group had a double upregulation in MuRF-1 and a moderate enhancement in atrogin-1; the BTX group had a nearly triple fold increase in atrogin-1/MuRF-1 (b, c) (∗p<0.05;∗∗p<0.01;∗∗∗p<0.001).
