Fig. 7.
Representative images from 72 h-incubated micropillar chips with 3D-cultured Hep3B cells, exposed to menadione (0.7 – 177 μM) for 48 h, and stained with the four fluorescent dyes: (A) TMRM for mitochondrial impairment, (B) Hoechst 33342 for DNA damage, (C) mBCl for changes in intracellular glutathione level, and (D) calcein AM for cell membrane integrity. The dose-dependent effect of menadione for all four assays was clearly observed from the microarray images obtained.