Capsaicin masseter injection increases time to puncture compared to baseline. Data for non-noxious and capsaicin masseter injections trials were normalized to individual animal baseline mean to adjust for variation among animal distance preference. A) No difference was found between baseline, isoflurane only, PBS injections, or vehicle injections (p>0.05). B) Capsaicin injection increased puncture time compared to baseline, PBS injections, and vehicle injections (p=0.0002, p=0.014, p=0.068). * indicates p<0.05. ^ indicates p<0.10.