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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Exp Neurol. 2018 May 2;306:92–104. doi: 10.1016/j.expneurol.2018.05.001

Figure 3. Disrupted myelination and nodes of Ranvier in the optic nerve of CNP;Cdk5 CKO mice.

Figure 3

(A–B) Reduction of MBP and PLP expression in optic nerve of 2-month-old CNP;Cdk5 CKO mice shown by immunostaining of MBP (A, red) and Western blot analysis (B) compared with controls. Quantitative analysis is shown below the gels. (C) EM and (D) G-ratio analysis reveals reduced myelin thickness in the optic nerve of 2-month-old CNP;Cdk5 CKO mice compared to controls. (E–H) Disorganized and reduced number of Caspr/Nav1.6 or Caspr/Kv1.1 pairs in the optic nerve of 2-month-old CNP;Cdk5 CKO compare to controls. (I–J) Confocal images showed disrupted organization and abnormal distribution of Nav1.6 and Kv1.1 in CNP;Cdk5 CKO (arrows) compared to control. Values are mean ± SEM. n = 4 animals per genotype. **P < 0.01. ***P < 0.001. In 3B, E–H: two-tailed unpaired Student’s t-test. Scale bars: B = 100 μm; D = 10μm.