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. 2018 Jun 4;9:663. doi: 10.3389/fphys.2018.00663

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Copyright © 2018 Li, Wang, Zhang, Xu, Cao and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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An odorant receptor specifically expressed in palps involved in detection of aldehydes. (A) Comparison of response level to hexanal in different organs and different genotypes by EPG or EAG. Abbreviations: 5A, the antenna of 5th instar nymph; 5MP, the maxillary palp of 5th instar nymph; WT, wild type; ds-GFP, dsRNA of GFP injected. The response of EPG or EAG was calculated from the response value of maxillary palp or antenna treated with chemicals minus the response value of maxillary palp or antenna treated with mineral oil as control. Error bar indicates SEM. ^∗p < 0. 05, one-way ANOVA with post hoc t-tests. (B) Comparison of response level to E-2-hexenal in different organs and different genotypes by EPG or EAG. Abbreviations are referred to (A). (C) Comparison of response level to hexanal in different genotypes by EPG. Abbreviations are referred to (A). ds-OR12, dsRNA of OR12 injected. (D) Comparison of response level to E-2-hexenal in different genotypes by EPG. Abbreviations are referred to (A). ds-OR12, dsRNA of OR12 injected. (E) Comparison of response level to (E,E)-2,4-heptadienal in different genotypes by EPG. (F) Comparison of response level to (E,E)-2,4-hexadienal in different genotypes by EPG. (G,H) Comparison of response level to hexanal or E-2-hexenal in different genotypes by EAG. (I) RNA silencing is checked after electrophysiological experiment with semi-quantitative RT-PCR. Actin was used to check template quality.