Figure 8.

The selective formyl peptide receptor 2 antagonist WRW₄ blocks the cooperation between SAA1(58–104) and CXCL8 in neutrophil chemotaxis. WKYMVm (10 ng/ml), CXCL8 (3 ng/ml), SAA1(58–104) (3,000 ng/ml), or a combination of CXCL8 and SAA1(58–104) were added to the lower compartment of the Boyden microchamber to measure neutrophil chemotaxis. WRW₄ (20 µg/ml) or buffer was added to the cells just before loading the cells in the upper compartment of the microchamber. Data represent the mean chemotactic index (CI) ± SEM and are pooled from four to six independent experiments. Statistically significant cooperation is indicated by a dagger (compared with the sum of the net CI values when both chemotactic agents are tested separately; ^†p < 0.05) and statistically significant inhibition by the antagonist is indicated by dollar signs (^$p < 0.05; Mann–Whitney U test).