Table 1.

Mass spectrometric analysis of the cleavage sites of matrix metalloproteinase-9 (MMP-9) in intact recombinant SAA1α.

a.a.	Intact serum amyloid A1 (SAA1) protein sequence
1–104b	RSFFSFLGEAFDGARDMWRAYSDMREANYIGS DKYFHARGNYDAAKRGPGG VWAAEAISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY
	COOH-terminal SAA1 peptide sequence	Rel. int. (%),a 3 h
52–104	VWAAEAISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY	29.9
57–104	AISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY	19.8
58–104	ISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY	50.3

^aRelative intensity (%) of the SAA1(52–104), SAA1(57–104), and SAA1(58–104) peptides after incubation for 3 h of intact SAA1α (Uniprot P0DJI8) with MMP-9 (E:S ratio 1:50) and subsequent analysis by mass spectrometry.

^bThe amino acid (a.a.) sequence of SAA1(1–51) is underlined in the intact protein sequence.