1.

A panel of miRNAs controls the expression of the immune checkpoints

Item	Tissue/cell line	Relationship to immune checkpoints	Function	Ref.
MiRNAs
MiR-424 (322)	Ovarian cancer tissue and ovarian cancer cell lines	Anticorrelates with CD80 and PD-L1	Low levels of miR-424(322) are associated with chemoresistance	26
MiR-15/16 family	Glioma mouse model	Correlates with PD-1, TIM-3, LAG-3	Low levels of miR-15a/16 prolongs mice survival	32
	MPM tissue and MPM cell lines	Anticorrelates with PD-L1	High PD-L1 is associated with low miR-15/16 levels and short overall survival	48
MiR-138	Glioma mouse model	Anticorrelates with PD-1, CTLA-4	High level of miR-138 inhibit tumor progression	33
	CRC patient samples and CRC cell lines	Anticorrelates with PD-L1	Low levels of miR-138 are associated with shorter overall survival	34
MiR-28	Exhausted T-cells from mice melanoma	Anticorrelates with PD-1, TIM3 and BTLA	Low levels of miR-28 induces T-cell exhaustion	35
MiR-155	Mouse T-cells	Anticorrelates with BTLA	Low levels of miR-155 decrease CD4+ T cell activation	37
	Dermal lymphatic endothelial cells	Anticorrelates with PD-L1	MiR-155 is part of a regulatory loop which controls the expression of PD-L1	57
MiR-29c and 　other 12 miRNAs	Breast cancer cell lines and tissue from breast cancer patients	Anticorrelates with B7-H3	High levels of miR-29c associate with a decreased risk of dying from breast cancer	39
MiR-570	Gastric cancer tissue	Anticorrelates with PD-L1	The inability of miR-570 to bind the PD-L1 mRNA leads to an aggressive gastric cancer phenotype	42
MiR-34a 　(and miR-34 family)	TCGA lung adenocarcinoma, p53 (R172HΔ)g/+K-ras (LA1/+) mouse model and various cell lines	Anticorrelates with PD-L1	P53 regulates the anti-tumor immunity by overexpressing miR-34, an inhibitor of PD-L1	43
	AML patient samples and leukemia cell lines	Anticorrelates with PD-L1	High levels of miR-34 decrease T-cell apoptosis	44
MiR-34a and 　MiR-200c	AML cell lines and AML mouse model	Anticorrelates with PD-L1	High levels of miR-34a and miR-200c leads to increased immune mediated killing of the tumor	45
MiR-197	NSCLC patient samples and human lung cancer cell lines	Anticorrelates with PD-L1	Low level of miR-197 predict low survival in NSCLC	46
	Oral squamous cell carcinoma	Anticorrelates with PD-L1	High levels of miR-197 predict poor overall survival	52
MiR-200	Lung adenocarcinoma databases, different mouse models and cell models	Anticorrelates with PD-L1	MiR-200 simultaneously inhibits neoplastic invasion and immunosuppression	49
MiR-20b, miR-21 　and miR-130b	CRC tissue	Correlate with PD-L1	MiR-20b, miR-21 and miR-130b inhibit PTEN, which is an inhibitor of PD-L1	50
MiR-574-3p	Spinal chordoma tissue	Anticorrelates with PD-L1	Low levels of miR-574-3p are associated with worse local recurrence-free survival	51
MiR-25-93-106b 　cluster	Primary pancreatic cancer cells from murine models	Anticorrelates with PD-L1	The miRNA cluster controls the bone marrow metastasis	53
Continued