FIG. 3.

Radioautographs and quantification of relative T4 associated with TBG, HSA, and TTR separated by non-denaturing PAGE in serum from (A) a WT individual with different amounts of added ¹²⁵I-labeled T4 and (B) serially diluted sera from a WT individual or an individual homozygous for the HSA mutation. (A) Sera are from aliquots containing 20,000 cpm (lane 1), 10,000 cpm (lane 2), or 5000 cpm (lane 3) of ¹²⁵I-labeled T4. (B) Sera was undiluted (lanes 1 and 4), diluted 1:2 (lanes 2 and 5), or diluted 1:4 (lanes 3 and 6), and 10,000 cpm (or 8 pg) of ¹²⁵I-labeled T4 was added to each sample. The relative amounts of T4 added with the ¹²⁵I-labeled T4 were 1.1–4.4% of the endogenous T4 for the WT and 0.3–1.2% for the mutant.