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. 2018 May 3;20(6):543–554. doi: 10.1016/j.neo.2018.04.002

Figure 3.

Figure 3

STEAP3 promotes GBM cell growth and GSC self-renewal. (A) STEAP3 knockdown efficiency as assessed by qRT-PCR in GBM#01 and GBM#P3 GSCs transfected with si-Ctrl or two different siRNAs targeting STEAP3 (si-STEAP3#1 and #2). GAPDH was used as the internal normalization control. (B) Cell growth curves generated for GBM#01 and GBM#P3 cells transfected in vitro with si-Ctrl and si-STEAP3 from data obtained using the Luminescent Cell Viability Assay kit. Measurements (luminescence) was obtained at days 1, 2, 3, and 4. (C) Representative images of tumor sphere formation for GBM#01 and GBM#P3 GSCs transfected with si-Ctrl and si-STEAP3. Scale bar = 100 μm. (D) Extreme limiting dilution assay performed with GBM#01 and GBM#P3 GSCs. GSCs were transfected with si-Ctrl and si-STEAP3, and analysis was performed 10 days after transfection. Stem cell frequency was calculated using ELDA. (E) Ectopic expression of STEAP3 in GBM#01 (GBM#01-STEAP3) confirmed by qRT-PCR. GAPDH was used as an internal normalization control. GBM#01-STEAP3 in tumor sphere formation assays (F) and ELDA (G). Data are shown as the mean ± SEM from three independent experiments. *P < .05; **P < .01; ***P < .001.