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Upregulation of angiogenic growth factors in 3D tumor clusters. (A and B) Bright-field micrographs of tumor cells in 3D tumor clusters after 24 hours of culture. Similar clusters were observed for all samples except B63, which were dispersed upon resuspension in the collagen. (C) Gene expression levels determined by quantitative RT-PCR. Error bars are the standard deviation of triplicate reactions. Scale bar, 100 μm. Asterisks indicate P < .01 (**) or P < .05 (*).
