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Steps in seeding microphysiological devices. Nortis single-channel microphysiological system containing ports for extracellular matrix collagen (red) and luminal flow (blue). b, bubble trap; i, injection port; v, valve. The red box shows the size of the region imaged (1024×1024 μm) in the panels in Figure 5 (the box is situated over the outlet region).
