Fig. 4. The effects of KY7749 on proliferation and levels of pan-Ras and p-ERK in CRC cells harboring WT and MT CTNNB1 and KRAS.

a Immunoblot analysis of SW480, DLD-1, HCT-116, and SW48 CRC cells treated with various doses of KY7749 for 24 h. WCLs were immunoblotted with antibodies against the indicated proteins. b MTT assays in SW480, DLD-1, HCT-116, and SW48 CRC cells cultured with various doses of KY7749 for 96 h (mean ± SD, n = 3). Relative cell proliferation was normalized to DMSO-treated controls and 50% growth inhibition (GI₅₀) was calculated