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. 2018 Jun 1;29(11):1376–1388. doi: 10.1091/mbc.E17-10-0594

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© 2018 Sundaram et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Effects of BiP depletion on IRE1α, PERK, and ATF6α complexes and their activation. (A) HEK293 cells were transfected with siRNA oligos directed against either BiP siRNA or Sec61α for two consecutive days. After 72 h of transfection, the cells were treated with 3.9 μM of TG for the indicated time points and analyzed by immunoblotting for the indicated antigens. (B–F) The samples from A were analyzed by BN–PAGE immunoblotting for the indicated antigens.