TABLE 2:
Cluster diameter.
| mEOS3.2-Cdc42 | WT | BEM1 | bem1Δ | |||||
|---|---|---|---|---|---|---|---|---|
| Pole (P) | Nonpole (NP) | P | NP | P | NP | P | NP | |
| Number of clusters | 113 | 171 | 239 | 654 | 137 | 324 | 132 | 583 |
| Median (nm; IQR) | 73 (51–95) | 56 (41–73) | 74 (48–103) | 62 (46–83) | 73 (48–101) | 63 (46–94) | 50 (37–72) | 52 (38–76) |
| Mean (nm; SEM) | 77 (±3.6) | 58 (±1.7) | 82 (±2.9) | 68 (±1.2) | 80 (±3.7) | 74 (±2.2) | 61 (±3) | 61 (±1.4) |
| bem1Δ + Lyso-PS | W303 | cdc42 Q61L | psd1Δpsd2Δ | |||||
|---|---|---|---|---|---|---|---|---|
| P | NP | P | NP | P | NP | P | NP | |
| Number of clusters | 126 | 696 | 137 | 272 | 306 | 130 | 174 | 227 |
| Median (nm; IQR) | 61 (44–97) | 62 (46–87) | 68 (48–97) | 50 (36–76) | 81 (62–115) | 70 (50–103) | 84 (56–124) | 60 (48–78) |
| Mean (nm; SEM) | 74 (±4) | 72 (±1.4) | 75 (±3.1) | 60 (±2) | 92 (±2.4) | 82 (±4.2) | 98 (±4.3) | 64 (±1.5) |
| WT + Lyso-PS | WT + Lyso-PC | cho1Δ | cho1Δ + Lyso-PS | ||||||
|---|---|---|---|---|---|---|---|---|---|
| P | NP | P | NP | All cell | P | NP | P | NP | |
| Number of clusters | 99 | 252 | 205 | 353 | 324 | 153 | 355 | 93 | 375 |
| Median (nm; IQR) | 89 (59–117) | 63 (48–81) | 71 (47–113) | 59 (46–75) | 52 (39–67) | 65 (43–94) | 60 (45–82) | 70 (42–99) | 61 (43–83) |
| Mean (nm; SEM) | 94 (±5) | 67 (±1.7) | 85 (±3.9) | 62 (±1.3) | 53 (±1.1) | 73 (±3.3) | 66 (±1.5) | 76 (±4.5) | 65 (±1.5) |
| cho1Δ+ Lyso-PC | PIL1-mEOS | ||
|---|---|---|---|
| P | NP | All cell | |
| Number of clusters | 135 | 462 | 586 |
| Median (nm; IQR) | 64 (48–85) | 64 (44–85) | 105 (79–141) |
| Mean (nm; SEM) | 68 (±2.4) | 68 (±1.4) | 115 (±2) |
The values displayed are for mEOS-Cdc42 clusters, with the exception of the first data set, which displays mEOS3.2-Cdc42 clusters, and the last data set, which displays Pil1-mEOS clusters.