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A) The reaction of sel-green probe with selenols. The fluorescence is released by the reaction of sel-green probe with selenol groups in K. brevis homogenate. B) Sel-green (4 µM) was incubated with K. brevis homogenate (0.6 mg protein/ml) and fluorescence was monitored (λex/em = 370/502 nm) at 0, 2.5 and C) 50 min. The fluorescence was normalized to protein concentration in the homogenate. High toxin (white bar) and low toxin (black bar) strains.
