Table 5.
Detection of polygalacturonase genes in transcriptomes and with PCR in genomic DNA of Sclerotinia sclerotiorum and S. trifoliorum
| Gene | Genomic location | PCR forward (F-) and reverse (R-) primer sequences (5′ to 3′)b | S. sclerotiorum | S. trifoliorum | ||
|---|---|---|---|---|---|---|
| Transcript (RPKM) | Genomic DNA | Transcript (RPKM) | Genomic DNA | |||
| SSPG1 | SS1G_01407 | F- TTTGGATCTTGGAAATGATG | JP575285 (396) | Y | JP557844 (217) | Y |
| R- GGTGGACCATTGTACTCAGA | ||||||
| SSPG1d | SS1G_10167 | F-CTAGTGGAATCCAGTGCTTG | JP592135(18) | Y | JP557846 (460) | Y |
| R-AGTACGGTGTTGTCATCGAG | JP580764(21) | |||||
| SSPG3 | SS1G_10698 | F- TACAGAGTCGGAGTTGGAAA | Not expressed | Y | Not expressed | Y |
| R- CGTCTTTGAGCTTTTGAAGA | ||||||
| SSPG5 | SS1G_04177 | F- TTCTCATTCCTTGGCTCATA | Not expressed | Y | Not expressed | Y |
| R- AAACGATGGTAGCACAAGAA | ||||||
| SSPG6 | SS1G_11057 | F- ATCAAGTCCAACGAAGGAAC | JP591710 (20) | Y | Not expressed | Y |
| R- CCGGTTGATGGATAGTTACA | JP584752(20) | Not expressed | ||||
| SSXPG1 | SS1G_04207 | F- TGCAAATAAATATGCGAGGT | JP586867 (23) | Y | JP567830 (24) | Y |
| R- GCCAGTAAAGGACCTGAAGT | ||||||
| SSXPG2 | SS1G_02553 | F- CTCCTGTGGTCAACAAACAT | Not expressed | Y | JP560305 (23) | Y |
| R- ATGTTTGTTGACCACAGGAG | ||||||
Y, positive detection by PCR in genomic DNA.