Fig. 1. Cyclic voltammograms (CVs) of acetylcholine (ACh) detection in vitro with the nanoITIES pipet electrode in the cellular medium of Aplysia, and artificial seawater (ASW). The electrochemical cell diagram is shown in cell 1. The CV of the ASW background solution is shown as an orange solid line, and the sigmoidal shape of the CV is due to the transfer of ASW ions facilitated by 1 mM DB18C6 inside the pipet (detailed current contributions of ASW ions are included in Fig. S3†). The CV for ACh detection is shown as a sigmoidal blue dashed line. The nanoelectrode radius is ∼370 nm. Cell 1: Pt|1 mM DB18C6 + 5 mM TDDATFAB + 1,2-DCE‖ASW|AgCl|Ag.
