Table I.
Primer sequences used in PCR reactions.
| Genes | Primer sequences (5′3′) | TM (°C) |
|---|---|---|
| HIF-1α | F:GAACCCATTTTCTACTCAGGACACAG | 55 |
| R:CCACTTTCATCCATTGATTGCCC | ||
| VEGF | F:CGAGACCCTGGTGGACAT | 58 |
| R:ACAAATGCTTTCTCCGCT | ||
| Ang-1 | F:AGACCAGTACAACACAAAC | 58 |
| R:CGGTCTGAGAGAGGAGGCT | ||
| TKLK | F:TCATCAATGAAGACCTATGCG | 56 |
| R:AGCATCAGGTCATTGCTGTA | ||
| PEDF | F:CTACAGGTGCGCGCCAAC | 61 |
| R:CTAAGGCGCACACATTTG | ||
| Bax | F:GCGAATTGGAGATGAACTGG | 56 |
| R:GTGAGCGAGGCGGTGAGGAC | ||
| Caspase-3 | F:GAAGCGAATCAATGGACTCTG | 58 |
| R:GCACAAAGCGACTGGATGAA | ||
| Bcl-2 | F:CTGGTGGACAACATCGCTCTG | 58 |
| R:GGTCTGCTGACCTCACTTGTG | ||
| Survivin | F:ATCCACTGCCCTACCGAGAAC | 58 |
| R:TGCTCCTCTATCGGGTTGTCAT | ||
| Nrf-2 | F:TCCTCTGCTGCCATTAGTCA | 58 |
| R:GTGCCTTCAGTGTGCTTCT | ||
| HO-1 | F:CAGGAGCTGCTGACCCATGA | 58 |
| R:AGCAACTGTCGCCACCAGAA | ||
| NQO-1 | F:GGATTGGACCGAGCTGGAA | 58 |
| R:AATTGCAGTGAAGATGAAGGCAAC | ||
| β-actin | F:GACCCAGATCATGTTTGAGACCTT | 58 |
| R:GACTCGTCATACTCCTGCTTGC |
F, forward; R, reverse; HIF-1α, hypoxia-inducible factor-1α; VEGF, vascular endothelial growth factor; Ang-1, angiopoietin; TKLK, tissue kallikrein; PEDF, pigment epithelium-derived factor; Bax, Bcl-2 related X protein; caspase-3, cysteine aspartate specific protease; Nrf-2, nuclear factor erythroid 2 (NFE2)-related factor 2; HO-1, heme oxygenase-1; NQO-1, NAD(P)H quinone dehydrogenase 1.