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. 2018 May 18;16(1):73–82. doi: 10.3892/etm.2018.6188

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of overexpression of GAS5. A2780 cells were transfected with GAS5 plasmid or blank vector. Non-transfected cells were used as the control group. Following transfection, (A) qPCR was used to examine GAS5 expression, (B) an MTT assay was used to determine cell proliferation, (C) qPCR was used to examine miR-21 levels and (D) western blot analysis was used to examine the protein levels of SPRY2. **P<0.01 vs. Control. GAS5, growth arrest-specific transcript 5; SPRY2, Sprouty homolog 2; qPCR, quantitative polymerase chain reaction; miR, microRNA.