Figure 5.

A2780 cells were co-transfected with GAS5 expression plasmid and SPRY2 siRNA or non-specific siRNA. Following transfection, (A) quantitative polymerase chain reaction and (B) western blot analysis were used to examine the mRNA and protein levels of SPRY2, respectively. (C) An MTT assay was used to determine cell proliferation. **P<0.01 vs. GAS5+siNC. siRNA, small interfering RNA; siNC, negative control siRNA; siSPRY2, SPRY2 siRNA; GAS5, growth arrest-specific transcript 5; SPRY2, Sprouty homolog 2.