Figure 2.

COL1A1 is a direct target of miR-29b. (A) The full-length 3′-UTR of COL1A1 mRNA contains three miR-29b-binding sites. (B) ASMCs were co-transfected with either miR-29b mimics or Scr-miR control, together with COL1A1-3′-UTR sequence. The relative luciferase activities were measured and normalized by Renilla luciferase value after 72 h. All data are presented as mean ± standard deviation. Similar results were obtained from 3 independent experiments. **P<0.01. (C) COL1A1 protein level in ASMCs cells co-transfected with either miR-29b mimics or Scr-miR control. 3′-UTR, 3′-untranslated region; ASMCs, aortic smooth muscle cells.