Table 1. Sequence statistics and alpha diversity of Vietnamese sponge-associated prokaryotic communities, including the total number of OTUs, coverage, and alpha diversity metrics.

Sample	Taxon	No. of OTUs	Coverage	Evenness	Shannon	Inverse Simpson
AMC	Amphimedon sp. 1	47	99.8	0.87	2.25	2.16
AMQ	Amphimedon sp. 2	31	99.8	0.84	2.14	2.90
AXT.1	Axinyssa sp.	94	99.6	0.94	4.95	18.52
AXT.2	Axinyssa sp.	107	99.5	0.94	4.98	17.86
AXT.3	Axinyssa sp.	85	99.6	0.94	4.70	15.15
AXT.4	Axinyssa sp.	99	99.6	0.94	4.84	15.87
AXC	Axos cliftoni	92	99.7	0.95	4.56	10.64
CIS	Cinachyrella schulzei	115	99.4	0.95	4.87	10.75
CLR.1	Clathria reinwardti	145	99.3	0.96	5.84	27.03
CLR.2	Clathria reinwardti	176	99.5	0.97	5.95	38.46
DAS.1	Dactylospongia sp.	157	99.2	0.96	5.81	30.30
DAS.2	Dactylospongia sp.	141	99.4	0.96	5.70	29.41
HAS	Halichondria sp.	108	99.5	0.94	4.63	11.24
HAA.1	Haliclona amboinensis	118	99.5	0.93	4.62	10.99
HAA.2	Haliclona amboinensis	92	99.5	0.93	4.33	9.26
HAF	Haliclona fascigera	89	99.6	0.93	4.77	15.39
CRV	Haplosclerida sp.	58	99.8	0.90	3.58	6.80
NIS	Niphatidae sp.	35	99.8	0.86	2.08	2.36
RHG	Rhabdastrella globostellata	146	99.3	0.96	5.36	15.63
SPV	Spheciospongia sp.	122	99.2	0.96	5.66	26.31
SPS.1	Spirastrella sp.	98	99.6	0.93	4.37	9.71
SPS.2	Spirastrella sp.	76	99.7	0.92	3.93	7.94
TES	Tedania sp.	165	99.9	0.97	6.04	40.00
TEA	Terpios aploos	19	99.9	0.79	2.12	3.47
XES.1	Xestospongia testudinaria	92	99.6	0.95	4.86	13.16
XES.2	Xestospongia testudinaria	121	99.6	0.95	4.96	12.35
XES.3	Xestospongia testudinaria	117	99.5	0.95	5.02	16.39

Note:

Alpha diversity values (evenness, Shannon, inverse Simpson) were calculated based on subsampling size of the sample with the fewest sequences (n = 1,595 reads).