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. Author manuscript; available in PMC: 2018 Jun 11.
Published in final edited form as: J Invest Dermatol. 2017 Jan 25;137(6):1322–1332. doi: 10.1016/j.jid.2016.12.026

Table 1.

Mass spectrometry analysis was used to identify the ubiquitination sites of ERK5

Conf Sc Prec m/z z Sequence Theor MW ΔMass Site
99 16 562.2949 3 GLK-ubY1 M 2 HASQVIHR 1,683.8628 −0.0293 K171
41.4 12 1008.999 2 DLK-ubP2 SNLLVNENC 3 ELK 2,015.9834 −0.0197 K184
44.1 14 816.8583 4 HPFLAK-ubY1 H 2 DPDDEPD C3 APPFDFAFDR 3,263.4043 −0.0269 K349

Abbreviations: ΔMass, the difference between theoretical MW and experimental MW of the matching peptide sequence; Conf, the confidence for the peptide identification; ERK5, extracellular signal-regulated kinase 5; K, lysine; Prec m/z, precursor m/z; Sc, the score for the peptide; Theor MW, theoretical precursor molecular weight for peptide sequence; z, the charge for the fragmented ion.

1

Tyrosine oxidation to 2-aminotyrosine.

2

Oxidation.

3

Carbamidomethyl.