Figure 2. Notochordal cell-derived matrix (NCM) induces extracellular matrix deposition by chondrocyte-like cells (CLCs).

Safranin O/Fast Green staining and collagen type I, II, and X immunohistochemistry of canine (A) and human (B) CLC micro-aggregates of 35,000 cells cultured in basal culture medium (negative control), supplemented with 10 ng/mL TGF-β₁ (positive control), and 10 mg/mL NCM for 28 days. n = 6 per species, in duplicates. All scale bars indicate 200 µm.