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. 2018 May 29;9(41):26507–26526. doi: 10.18632/oncotarget.25476

Figure 3. Notochordal cell-derived matrix (NCM) facilitates chondrogenic differentiation of canine MSCs.

Figure 3

DNA (A), glycosaminoglycan (GAG; B) and GAG/DNA (C) content. MSC micro-aggregates of 35,000 cells were cultured in (negative) control culture medium, supplemented with 10 ng/mL TGF-β1 (positive control) or 10 mg/mL NCM for 28 days. (D) Safranin O/Fast Green staining and collagen type I, II, and X immunohistochemistry at day 28. *,***p < 0.05, p < 0.001, respectively, for the indicated condition versus controls; #,##p < 0.05 and p < 0.01, respectively, for the two indicated conditions by the horizontal bar; n = 3 in duplicates; mean values are given. ANOVA (normally distributed data), Kruskal Wallis/Mann–Whitney U (non-parametric data), and Benjamini & Hochberg tests were performed.