Table 4.

Hydrolysis kinetic assays of reactor biomass at 37°C and 12°C, based on a skimmed milk protein source. a; Maximum substrate utilising rate gCOD gProtein⁻¹ d⁻¹. b; Apparent half-saturation rate constant gProtein l⁻¹. c; Maximum initial velocity gProtein l⁻¹ d⁻¹ for R1 and R2, d: Hydrolysis rate constant d⁻¹. Values are the mean of triplicates ± standard deviation in brackets.

Sample	Amaxa	Kmb	Vmax c	kd
Seed 37°C	15 (±3)	1.1 (±0.41)	0.9 (±1.07)	0.9 (±0.58)
Seed 12°C	18 (±0.13)	2.5 (±0.03)	0.3 (±0.05)	0.3 (±0.06)
R1 Phase 1 37°C	12 (±1)	1.8 (±0.36)	2.2 (±1.13)	1 (±0.42)
R2 Phase 1 37°C	104 (±10)	2.7 (±0.07)	0.8 (±0.23)	0.8 (±0.31)
R1 Phase 1 12°C	40 (±7)	1 (±0.34)	1.1 (±0.4)	1.3 (±0.06)
R2 Phase 1 12°C	19 (±2.52)	4.3 (±3)	2.7 (±2.19)	0.9 (±0.21)
R1 Phase 2 37°C	145 (±26.5)	3.9 (±2.12)	8.8 (±8.39)	5.7 (±2.18)
R2 Phase 2 37°C	164 (±16)	3.1 (±2.23)	7.5 (±6.72)	2.1 (±0.91)
R1 Phase 2 12°C	127.5 (±45)	1.6 (±0.11)	0.1 (±0.04)	0.8 (±0.16)
R2 Phase 2 12°C	35 (±27)	1.4 (±0.13)	0.1 (±0.06)	1.2 (±0.58)
R1 Phase 3 37°C	94 (±26)	1.6 (±0.43)	3.3 (±3.94)	1.6 (±0.37)
R2 Phase 3 37°C	62 (±9.5)	2.8 (±0.14)	3.1 (±0.70)	2.2 (±0.41)
R1 Phase 3 12°C	52 (±32)	2.6 (±0.19)	0.3 (±0.09)	1.1 (±0.32)
R2 Phase 3 12°C	91 (±29)	1.9 (±0.22)	0.3 (±0.16)	1.5 (±0.33)
R1 Phase 4 37°C	257 (±32)	1.8 (±0.27)	1 (±1.07)	0.9 (±0.58)
R2 Phase 4 37°C	15 (±1)	2.1 (±2.02)	2 (±2)	1.9 (±1.18)
R1 Phase 4 12°C	40 (±18)	0.5 (±0.04)	0.1 (±0.01)	3.2 (±0.89)
R2 Phase 4 12°C	51 (±5)	0.3 (±0.08)	1.7 (±1.57)	4.7 (±2.92)
R1 End 37°C	89 (±33)	2.8 (±1.58)	2.3 (±3.73)	1.4 (±0.787)
R2 End 37°C	72 (±8)	1.2 (±0.16)	1 (±0.27)	2.9 (±0.216)
R1 End 12°C	68 (±7)	1.8 (±0.37)	0.4 (±0.23)	1.8 (±0.714)
R2 End 12°C	83 (±22)	2.2 (±0.19)	0.6 (±0.15)	1.6 (±0.383)