Figure 5. Fap1-inhibition decreases abundance of CD133⁺CD44⁺ cells in in a murine xenograft model of colon cancer with or without oxaliplatin.

Tumors from the mice described above were analyzed for cell population distribution after various treatments. (A) Histograms from flow cytometry demonstrate decreased abundance of CD133⁺CD44⁺ cells after treatment with SLV peptide with or without oxaliplatin. A representative histograms for each cohort is shown. (B) Treatment with SLV peptide decreases relative abundance of CD133⁺CD44⁺ cells in xenograft tumors. Tumors were simultaneously harvested from mice treated with SLV peptide versus VLS control (when control group tumors were >2,000 mm³) and analyzed for CD133 and CD44 expression by flow cytometry. Significant differences indicated by ^*, ^**, or ^***. (p<0.001, n=6). (C) The addition of SLV peptide to oxaliplatin treatment decreases relative abundance of CD133⁺CD44⁺ cells in xenograft tumors. Tumors were simultaneously harvested from mice treated with oxaliplatin + SLV peptide versus VLS control (when control group tumors were >2,000 mm³) and analyzed for CD133 and CD44 expression by flow cytometry. Significant differences indicated by ^*, ^**, or ^***. (p<0.001, n=6).