Figure 2. Expression of molecules involved in the p53 and autophagy pathways.

(A, D) JMN-1B (A) or AsPC-1 (D) cells were treated with 17-AAG (1 μM), 17-DMAG (0.1 μM), Ad-p53 (3 × 10⁴ vp/cell), Ad-LacZ (3 × 10⁴ vp/cell) or combination as indicated for 48 hours. JMN-1B cells expressed MDM2 predominantly at 60 kDa. Actin was used as loading control. (B, C, E, F) Representative immunofluorescence staining of JMN-1B (B, C) or AsPC-1 (E, F) cells with anti-p53 Ab under the same imaging condition. Cells treated with 17-AAG (1 μM) or 17-DMAG (0.1 μM), or with Ad-p53 (3 × 10⁴ vp/cell) together with 17-AAG (1 μM) or 17-DMAG (0.1 μM), were stained with anti-p53Ab, (B, E) Pab1620 or (C, F) DO-1, and PI.