Skip to main content
. 2018 May 25;9(40):26019–26031. doi: 10.18632/oncotarget.25419

Figure 2. Effect of different cytokines on CLL apoptosis and proliferation.

Figure 2

Twelve progressive CLL frozen samples (7 IGHV mutated and 5 unmutated) were used to test the effect of 26 different cytokine conditions with media containing a pool of 3 progressive CLL NE and human serum. (Panel A) shows the results of the 3 backbone stimulation (sCD40L+CpG, sCD40L+IL-21 and CpG+IL-2) in terms of apoptosis and proliferation reflecting that CpG+IL2 provided better results. (Panel B) shows the incorporation of additional stimuli (BAFF, anti-BCR, sCD40L, HS5) to the CpG+IL2 backbone stimulation reflecting that the use of CpG+IL2 and HS5 (at 1:100 ratio) showed the best effects with a median of 30 ± 19% of apoptosis and 30 ± 27% of proliferation. Data from apoptosis are subtracted from the value of apoptosis after thaw.