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. 2018 Apr 5;10(5):1551–1564. doi: 10.1016/j.stemcr.2018.03.005

Figure 1.

Figure 1

Screening of AAV Serotypes

(A) Route of injection.

(B) Macroscopic appearance of wild-type mouse testes 1 week after microinjection with mCherry-expressing AAVs.

(C) Immunostaining of AAV1, AAV8, or AAV9-mCherry-injected testes for undifferentiated spermatogonia (GFRA1) and Sertoli cell (WT1) markers 1 week after microinjection with AAV-mCherry. Arrowheads indicate target cells infected with AAVs.

(D) Quantification of immunostaining. Three tubules from three different testes were counted for tubular and interstitial injection.

(E) Immunostaining of AAV1 or AAV9-mCherry-injected testes for STAR or ACTA2 1 week after microinjection with AAV-mCherry. Arrowheads indicate target cells infected with AAVs.

Error bars represent standard errors. Scale bars, 1 mm (B) and 40 μm (C and E). Counterstain, Hoechst 33342 (C and E). See also Figures S1–S6 and Table S1.